Table 1.
A compilation of all the site mapped N-linked glycosylation sites within the consensus sequence NXS/T found on proteins in membrane fractions of MCF-7, MDA-MB-453, and MDA-MB-468 breast cancer cell lines (#=deaminated asparagine, @=carboxyamidomethylation, *=oxidation, z=charge state). Only peptides with good Xcorrelation numbers (good XC: +1=1.5, +2=2.0, +3=2.5 and rigorous XC +1=1.9, +2=2.5, +3=3.5) were chosen and manually inspected.
| N-linked glycosylated proteins | Breast cancer cell lines | MH+ | z | Peptide | XC | Ions |
|---|---|---|---|---|---|---|
| galectin 3 binding protein | MCF-7, MDA-MB-453, MDA-MB-468 | 1348.68556 | 2 | R.ALGFEN#ATQALGR.A | 4.17 | 20/24 |
| oxygen regulated protein | MCF-7, MDA-MB-453, MDA-MB-468 | 1819.92586 | 2 | R.LSALDNLLN#HSSM*FLK.G | 3.50 | 16/30 |
| lysosome associated membrane protein 1 | MCF-7, MDA-MB-453, MDA-MB-468 | 1160.6059 | 2 | R.GHTLTLN#FTR.N | 2.66 | 15/18 |
| cathepsin D preproprotein | MCF-7, MDA-MB-453 | 1110.57896 | 2 | K.GSLSYLN#VTR.K | 3.75 | 15/18 |
| clusterin isoform 2 | MCF-7, MDA-MB-453 | 1684.81769 | 2 | R.LAN#LTQGEDQYYLR.V | 4.94 | 22/26 |
| pigment epithelium-derived factor | MCF-7, MDA-MB-453 | 2944.51497 | 4 | K.VTQN#LTLIEESLTSEFIHDIDRELK.T | 5.45 | 44/144 |
| breast cancer 1, early onset isoform 1 | MCF-7 | 1392.61251 | 2 | K.EEN#QGKN#ESN#IK.P | 2.16 | 13/22 |
| Integrin alpha-2 | MCF-7 | 1646.79483 | 2 | R.YFFN#VSDEAALLEK.A | 4.23 | 22/26 |
| leucine-rich alpha-2-glycoprotein 1 | MCF-7 | 1553.94138 | 2 | R.KLPPGLLAN#FTLLR.T | 2.64 | 13/26 |
| solute carrier family 3 | MCF-7 | 2323.14523 | 2 | -.DIENLKDASSFLAEWQN#ITK.- | 5.38 | 22/38 |
| Transforming growth factor beta-1 | MCF-7 | 2761.44069 | 3 | R.LASPPSQGEVPPGPLPEAVLALYN#STR.D | 4.17 | 29/104 |
| thrombospondin 1 | MCF-7 | 1367.69137 | 2 | K.VVN#STTGPGEHLR.N | 2.21 | 13/24 |
| Na+/K+ -ATPase beta 1 subunit | MCF-7 | 2383.22138 | 2 | K.YLQPLLAVQFTN#LTM*DTEIR.I | 3.25 | 16/38 |
| purinergic receptor P2X4 | MCF-7 | 1106.60920 | 2 | K.AAEN#FTLLVK.N | 3.19 | 15/18 |
| chromosome 20 open reading frame 3 | MDA-MB-453 | 1424.70562 | 2 | R.AGPN#GTLFVADAYK.G | 3.86 | 18/26 |
| lysosomal-associated membrane protein 2 | MDA-MB-453 | 1883.96905 | 2 | K.IAVQFGPGFSWIAN#FTK.A | 4.81 | 24/32 |
| prosaposin | MDA-MB-453 | 2783.35430 | 3 | K.DVVTAAGDM*LKDN#ATEEEILVYLEK.T | 4.01 | 27/96 |
| zinc finger protein 92 (HTF12) isoform 2 | MDA-MB-453 | 1668.77584 | 2 | -.C@GNAFN#QSSNLTARK.- | 2.61 | 10/28 |
| haptoglobin | MDA-MB-453 | 1795.99526 | 3 | K.VVLHPN#YSQVDIGLIK.L | 3.58 | 24/60 |
| haptoglobin | MDA-MB-453 | 2680.37631 | 3 | K.MVSHHN#LTTGAYLINEQWLLTTAK.N | 4.40 | 36/92 |
| clusterin | MDA-MB-453 | 1684.81769 | 2 | R.LAN#LTQGEDQYYLR.V | 4.74 | 22/26 |
| tissue inhibitor of metalloproteinases 1 | MDA-MB-453 | 1753.87554 | 2 | K.FVGTPEVN#QTTLYQR.Y | 3.73 | 20/28 |
| zinc-alpha-2-glycoprotein | MDA-MB-453 | 2067.92540 | 3 | K.DIVEYYN#DSN#GSHVLQGR.F | 2.76 | 20/68 |
| zinc-alpha-2-glycoprotein | MDA-MB-453 | 3417.59034 | 4 | K.AREDIFM*ETLKDIVEYYN#DSN#GSHVLQGR.F | 4.71 | 41/168 |
| CD44 antigen (PGP-1) | MDA-MB-468 | 1601.71857 | 2 | K.AFN#STLPTM*AQM*EK.A | 4.51 | 20/26 |
| human epidermal growth factor receptor | MDA-MB-468 | 1176.61066 | 2 | K.DSLSIN#ATNIK.H | 3.42 | 18/20 |
| intercellular adhesion molecule 1 ICAM-1 | MDA-MB-468 | 1614.76459 | 2 | R.LNPTVTYGN#DSFSAK.A | 3.99 | 20/28 |
| intercellular adhesion molecule 1 ICAM-1 | MDA-MB-468 | 999.61971 | 2 | R.AN#LTVVLLR.G | 2.41 | 12/16 |
| oxygen regulated protein | MDA-MB-468 | 1194.63648 | 2 | R.VFGSQN#LTTVK.L | 3.85 | 16/20 |