Table 1.
Primers employed in the CYP1A, CYP1B1, CYP1C1, CYP1C2, CYP1D1 and β-actin qPCR reactions in Fundulus heteroclitus
| primer name | Primer sequence 5′ - 3′ | Location |
|---|---|---|
| 1A forward | CTTTCACAATCCCACACTGCTC | 1301 - 1322 |
| 1A reverse | GGTCTTTCCAGAGCTCTGGG | 1404 - 1423 |
| 1B1 forward | ATATTTGGAGCCAGCCAGGAC ACG |
629 - 652 |
| 1B1 reverse | CGCACCTGCATCTCAGGGTACT TG |
691 - 714 |
| 1C1 forward | TCTGGACGCCTTCATCTACGA | 1296 - 1316 |
| 1C1 reverse | GTGACGTCCGATGTGGTTGA | 1360 - 1379 |
| 1C2 forward | GCAGGCTGCCATCTGTTGAGGA CA |
1257 - 1280 |
| 1C2 reverse | CGAAGCTGGTGAAACGCATTGT CT |
1317 - 1340 |
| 1D1 forward | CTTTCACCATCCCTCACTGCAC CA |
1322 - 1345 |
| 1D1 reverse | GTCTCCGGATCACCCCAAAGAT CC |
1428 - 1451 |
| β-actin forward | TGGAGAAGAGCTACGAGCTCC | * |
| β-actin reverse | CCGCAGGACTCCATTCCGAG | * |
*
no full sequence available