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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Cell Host Microbe. 2009 Jul 23;6(1):91–98. doi: 10.1016/j.chom.2009.05.018

Figure 2.

Figure 2

Reduced virus titers and myocarditis in Hrt-KO mice. A) Mice were infected with 1×105 PFU/mouse of CVB3-H3, and sacrificed 7 days post-infection. Each symbol represents the titer (PFU per gram of tissue) from an individual mouse. Means for each experimental group are indicated by horizontal black lines (n= 8 for each genotype). B) Serial titers in mice infected with 1×105 PFU (n=3–5 mice per experimental group). C) Hematoxylin and Eosin (H & E, top) and Masson’s Trichrome stain (bottom) of heart tissue sections. Uninfected samples are shown in the left-hand panels; sections from infected mice (day 7) are shown on the right. Scale bars, 80 microns. D) Severity scores for myocarditis and pancreatitis in control (n=8) and Hrt-KO mice (n=8), assessed as described in Experimental Procedures. E) Differential induction of cytokine and chemokine expression in heart tissue 7 days post infection (1×105 PFU), measured by quantitative RNA PCR. Expression is measured relative to uninfected control mice of the same genotype. Error bars are SEM, and asterisks indicate a statistical difference between the Hrt-KO and controls (*, p<0.05; ** p<0.005; #, p= 0.058).