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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Anal Biochem. 2009 Jun 30;394(1):39–47. doi: 10.1016/j.ab.2009.06.037

Fig. 2. Fluorescence resonance energy transfer assay for phosphopantetheinyl transferase.

Fig. 2

Action of PPTase on a fluorescently labeled acceptor substrate 5, in conjunction with a fluorphore-modified coenzyme A analogue (mCoA) 6, assembles a FRET pair upon conversion to the thiol-blocked crypto-acceptor substrate 7. The assembly of this FRET pair can be detected with a fluorescence microplate reader.