Table 1.

Summarized assay protocol

step	parameter	value	description
1	compound library	2.5µL	transfer to COSTAR 3694 black 96 well plate; Row G & H = DMSO for control
2	Enzyme solution	37.5µL	1.33X solution: 1.33mg/mL BSA, 66.6mM NaHEPES, pH 7.6, 13.3mM MgCl2,
3	time	15 min	Room tempertature incubation
4	substrate reagent	10µL	5X solution: 25µM FITC-YbbR 8, 50µM TAMRA-mCoA 12 in 10mM sodium phosphate
5	detection	Ex 485, Em 535 nm	Perkin-Elmer HTS7000 plus, kinetic read, once evey 2 min for 15 cycles