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. Author manuscript; available in PMC: 2009 Oct 13.
Published in final edited form as: FEMS Microbiol Lett. 2009 May 1;295(2):245–250. doi: 10.1111/j.1574-6968.2009.01602.x

Figure 3. Plc1p is not required for Mcm1p stability.

Figure 3

(A) Wild-type, ipk2Δ, and plc1Δ cells expressing TAP-tagged Mcm1p were grown in YPD medium to early exponential phase (A600nm = 1.0) and subsequently treated with cycloheximide to block protein synthesis. Mcm1p-TAP levels were determined by Western blotting using anti-TAP antiobody before addition of cycloheximide and after 1 and 3 h. Even loading of protein samples was confirmed with anti-3-phosphoglycerate (Pgk1p) antibody. The experiment was performed three times, and representative results are shown. (B) Densitometric evaluation of the representative Western blot.