Table 1.
a) The masses (Calculated and Found) for Fmoc-protected glycoamino acids and glycopeptides as 2D-HPLC fractions obtained from horseradish peroxidase, their proposed compositions and relative abundances. All the masses are sodium adducts ([M+Na]+) except fractions 1 & 2, which are proton adducts ([M+H]+). Green = fucosylated structures, Yellow = xylosylated structures, White = fucosylated and xylosylated structures, Blue = high mannose structures;b) The relative abundances of fucosylated, xylosylated, both fucosylated and xylosylated, and high-mannose glycan structures from HRP.
| a) | ||||
|---|---|---|---|---|
| 2D fraction No. | Mass (Found) | Mass (Calc.) | Proposed composition | Relative abundance (%) |
| 1 | 1681.75 | 1681.55 | Hex3HexNAc2Fuc1 Xyl1 -NR | 1.8 |
| 2 | 1681.68 | 1681.55 | Hex3HexNAc2Fuc1 Xyl1 -NR | 0.3 |
| 3 | 1401.54 | 1401.37 | Hex3HexNAc2Xyl1-N | 3.0 |
| 4 | 1646.72 | 1646.49 | Hex3HexNAc2Fuc1 Xyl1 -NV | 0.4 |
| 5 | 1458.60 | 1458.39 | Hex3HexNAc2Xyl1-GN | 1.2 |
| 6 | 1415.59 | 1415.43 | Hex3HexNAc2Fuc1-N | 0.9 |
| 7 | 1401.53 | 1401.37 | Hex3HexNAc2Xyl1-N | 3.9 |
| 8 | 1486.65 | 1486.46 | Hex3HexNAc2Fuc1-NA | 0.4 |
| 9 | 1634.63 | 1634.46 | Hex3HexNAc2Fuc1 Xyl1 -NS | 5.3 |
| 10 | 1648.67 | 1648.47 | Hex3HexNAc2Fuc1Xyl1-NT | 6.3 |
| 11 | 1547.63 | 1547.43 | Hex3HexNAc2Fuc1Xyl1-N | 32.0 |
| 12 | 1731.74 | 1731.55 | Hex3HexNAc2Fuc1Xyl1-LNA | 6.2 |
| 13 | 1849.82 | 1849.57 | Hex3HexNAc3Fuc1Xyl1-NV | 0.7 |
| 14 | 1634.53 | 1634.46 | Hex3HexNAc2Fuc1Xyl1-NS | 3.8 |
| 15 | 1603.86 | 1604.45 | Hex3HexNAc2Fuc1Xyl1-GN | 0.5 |
| 16 | 1648.63 | 1648.47 | Hex3HexNAc2Fuc1Xyl1-NT | 8.1 |
| 17 | 1547.59 | 1547.43 | Hex3HexNAc2Fuc1 Xyl1 -N | 4.4 |
| 18 | 1731.75 | 1731.55 | Hex3HexNAc2Fuc1Xyl1-LNA | 1.8 |
| 19 | 1804.71 | 1804.56 | Hex3HexNAc2Fuc1 Xyl 1 -SVNA | 0.4 |
| 20 | 1802.74 | 1802.55 | Hex3HexNAc2Fuc1Xyl1-SPNA | 1.2 |
| 21 | 1675.63 | 1675.49 | Hex3HexNAc2Fuc1Xyl1-NQ | 0.7 |
| 22 | 1634.62 | 1634.46 | Hex3HexNAc2Fuc1 Xyh -NS | 5.7 |
| 23 | 1807.69 | 1807.53 | Hex3HexNAc3Fuc1 Xyl1 -NG | 1.2 |
| 24 | 1750.68 | 1750.51 | Hex3HexNAc3Fuc1Xyl1-N | 4.6 |
| 25 | 1842.63 | 1842.56 | Hex6HexNAc2-NS | 1.2 |
| 26 | 1912.71 | 1912.56 | Hex4HexNAc3Fuc1 Xyl1 -N | 0.9 |
| 27 | 2004.70 | 2004.61 | Hex7HexNAc2-NS | 2.4 |
| 28 | 1917.72 | 1917.58 | Hex7HexNAc2-N | 1.0 |
| b) | |
|---|---|
| N-glycan Category | Molar Percentage(%) |
| Fucosylated only | 1.3 |
| Xylosylated only | 8.1 |
| Both fucosylated and xylosylated | 86.1 |
| High-mannose | 4.5 |