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. Author manuscript; available in PMC: 2009 Oct 13.
Published in final edited form as: Circ Res. 2009 Jan 8;104(4):496–505. doi: 10.1161/CIRCRESAHA.108.187567

Figure 3.

Figure 3

AMPK inhibition attenuates eNOS Ser635 phosphorylation in ECs. A, BAECs were pretreated with compound C at 20 μmol/L for 30 minutes before undergoing 30-minute treatment with laminar shear stress at 12 dyn/cm2, atorvastatin at 1 μmol/L, or adiponectin at 30 μg/mL. In B, HUVECs were transfected with AMPKα1 siRNA, AMPKα2 siRNA, or scramble RNA (10 nmol/L) before atorvastatin treatment. Phosphorylation of eNOS Ser635, AMPK Thr172, and ACC Ser79 was revealed by Western blotting. The bar graphs represent the densitometry analyses of Western blotting as means±SD of 3 or 4 independent experiments. *P<0.05 between groups as indicated.