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LC/MS/MS analysis of eNOS Ser635 and Ser1179 phosphorylation in BAECs. eNOS immunoprecipitated from AICAR-treated BAECs was trypsin-digested and then passed through TiO2- coated magnetic beads to enrich phosphopeptides. Nano-LC/MS/MS was used to map the phosphorylation site within the peptides containing Ser635 (A) or Ser1179 (B).
