Fig. 2.

Many inter-epithelia filopodia originate in the lens pit. (A-K) Eye region cryosections from E10.5 (A,B,D-K) or E9.5 (C) mouse embryos labeled for F-actin (phalloidin, green), nuclei with Hoechst 33258 (blue) and DiI in the lens pit (A,B, red), GFP from the Le-Cre transgene (C,D, red), β-catenin (E-H, red, white), tubulin (I, red), acetylated tubulin (J, red) or keratin 18 (K, red). The position of filopodia is indicated by white arrowheads; red arrowheads indicate filopodial position according to F-actin labeling. A gray line between panels indicates that they are separated color channels of the same image. Scale bars: 20 μm.