Fig. 4.

Inhibition of notochord activity of the Ci-tune CRM by repressor forms of Ci-Bra and Ci-FoxA-a. (A) Late-tailbud embryo co-electroporated with the 155-bp notochord CRM and with the Bra>Bra::en^RD construct, which directs notochord expression of Ci-Bra fused to the repression domain of Drosophila Engrailed (en^RD); stained for β-galactosidase (blue). (B) Late-tailbud stage embryo co-electroporated with the 155-bp notochord CRM and a repressor form of Ci-FoxA-a under the control of the Ci-tropomyosin-like (Ci-trop) notochord promoter (trop>FoxA-a::en^RD); stained for β-galactosidase (blue). Purple arrowheads indicate mesenchyme cells.