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. 2009 Oct 9;136(21):3679–3689. doi: 10.1242/dev.038141

Fig. 7.

Fig. 7.

Effects of changes in the spacing of the binding sites on notochord activity of the Ci-tune CRM. Late-tailbud embryos electroporated at the one-cell stage with either the wild-type 155-bp Ci-tune CRM or with versions containing insertions or deletions, schematized to the left of each micrograph. Embryos are stained for β-galactosidase (blue). (A) Wild-type Ci-tune CRM with the two Ci-Bra binding sites with identical core sequences spaced by 14 bp, and the Ci-FoxA-a binding site separated from T-box site 2 by 26 bp. (B) Deletion of 5 bp within the 14-bp spacer between the Ci-Bra binding sites. (C) Insertion of 10 bp in the 14-bp spacer. (D) Deletion of 5 bp between the T-box site 2 and the Ci-FoxA-a binding sites. (E) Insertion of 10 bp between the T-box site 2 and the Ci-FoxA-a binding sites nearly abolishes notochord activity, which is detected in a few cells in ∼9% of the transgenic embryos.