Figure 1.

Cisplatin induction and stabilization of p53 in teratocarcinoma cells. (a) F9 cells were treated with 10 μM cisplatin and whole protein extracts were prepared at the times indicated. Immunoprecipitation followed by Western blot analysis was performed as described in Materials and Methods. (b) The F9 cells were pulse-labeled as described in Materials and Methods, followed by exposure to cisplatin or cycloheximide (chx, 50 μg/ml) as indicated. (c) Cisplatin was added to the media 24 h before the pulse. Less total protein was immunoprecipitated for the time course of the cells treated with 10 μM cisplatin.