Figure 2.

Cisplatin induction of p21, but not cyclin G or mdm-2, in a p53-independent manner. The p53-wild-type (F9, Nulli-SCC1), and p53-mutant (EB16) teratocarcinoma cell lines were treated with 10 μM cisplatin. Total RNA was isolated at the indicated time points; 12 μg of RNA was resolved on a 1.1% agarose-formaldehyde gel, transferred to nitrocellulose, and probed with randomly primed cDNA. Two different blots were prepared from the same samples of RNA, one was probed with the p21, p53, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes, the other with the cyclin G and mdm-2 genes. The asterisk indicates the truncated p53 gene from the p53-mutant mouse.