Table 1.
Results of B. cinerea proteins with signal P identified in all three growth conditions.
| Gene Ida | Putative Functionb | Signal Pc | PE pectin | HE pectin | sucrose | |||
|---|---|---|---|---|---|---|---|---|
| Scored | Spectrae | Scored | Spectrae | Scored | Spectrae | |||
| BC1G_13137.1 | Polygalacturonase | 0.986Y | 492.13 | 12 | 791.28 | 16 | 73.04 | 1 |
| BC1G_00617.1 | Pectin methylesterase | 1.000Y | 247.49 | 6 | 296.65 | 11 | 122.37 | 2 |
| BC1G_06546.1 | alpha-galactosidase precursor (Melibiase) | 0.999Y | 247.44 | 7 | 47.29 | 1 | 129.34 | 2 |
| BC1G_07622.1 | beta-glucosidase | 0.995Y | 523.25 | 13 | 396.44 | 5 | 461.52 | 11 |
| BC1G_07319.1 | 1,3-beta glucanase | 0.999Y | 792.22 | 16 | 808.75 | 17 | 532.25 | 9 |
| BC1G_10455.1 | 1,3-beta-glucanosyltransferase Gel1 | 0.998Y | 254.54 | 4 | 172.44 | 3 | 263.19 | 10 |
| BC1G_12859.1 | alpha-glucosidase precursor | 1.000Y | 443.98 | 10 | 559.85 | 10 | 552.39 | 19 |
| BC1G_04994.1 | alpha-L-arabinofuranosidase | 0.999Y | 816.83 | 15 | 460.12 | 10 | 50.56 | 2 |
| BC1G_08372.1 | alpha-L-arabinofuranosidase A | 0.995Y | 100.63 | 2 | 336.39 | 4 | 102.4 | 1 |
| BC1G_06328.1 | alpha-L-rhamnosidase | 0.992Y | 218.77 | 6 | 265.45 | 5 | 231.9 | 6 |
| BC1G_14030.1 | beta-1-3-glucanosyltransferase | 1.000Y | 409.21 | 12 | 525.03 | 10 | 420.58 | 9 |
| BC1G_10247.1 | beta-fructofuranosidase | 1.000Y | 518.21 | 11 | 331.52 | 6 | 57.64 | 2 |
| BC1G_03567.1 | beta-galactosidase | 0.998Y | 792.51 | 17 | 309.73 | 6 | 266.3 | 6 |
| BC1G_02364.1 | beta-glucosidase | 0.991Y | 608.27 | 16 | 602.61 | 9 | 273.37 | 5 |
| BC1G_10221.1 | beta-glucosidase 1 precursor | 0.816Y | 1203.24 | 29 | 651.09 | 13 | 1544.26 | 48 |
| BC1G_13690.1 | Cell wall glycosyl hydrolase YteR, putative | 0.998Y | 243.78 | 4 | 495.08 | 13 | 110.69 | 3 |
| BC1G_06509.1 | Chitin binding protein | 1.000Y | 307.19 | 5 | 308.5 | 4 | 572.64 | 11 |
| BC1G_00594.1 | Endoglucanase | 0.999Y | 586.93 | 28 | 516.98 | 26 | 290.77 | 9 |
| BC1G_13938.1 | Exo-arabinanase | 1.000Y | 396.29 | 10 | 604.94 | 15 | 92.18 | 2 |
| BC1G_01033.1 | Exo-beta-1,3-glucanase | 0.929Y | 69.41 | 1 | 137.36 | 3 | 67.03 | 1 |
| BC1G_01617.1 | Exo-polygalacturonase | 0.996Y | 1098.98 | 29 | 833.38 | 33 | 116.36 | 2 |
| BC1G_07215.1 | Family 20 chitobiase | 0.999Y | 170.5 | 3 | 99.4 | 2 | 141.94 | 3 |
| BC1G_11018.1 | Family of alpha-1,6-mannanases | 0.999Y | 480.62 | 7 | 411.05 | 9 | 631.97 | 11 |
| BC1G_00409.1 | Glcosyl transferase/cell wall glucanase | 1.000Y | 347.29 | 6 | 271.42 | 7 | 246.29 | 4 |
| BC1G_08755.1 | Glucoamylase P precursor | 0.997Y | 359.15 | 7 | 126.38 | 2 | 366.87 | 6 |
| BC1G_11898.1 | Glucosidase | 0.999Y | 507.88 | 12 | 767.12 | 17 | 748.96 | 12 |
| BC1G_12132.1 | Glycosyl hydrolase family 65 protein | 0.997Y | 152.95 | 4 | 186.69 | 4 | 286.58 | 5 |
| BC1G_09079.1 | GPI-anchored cell wall beta-1,3-endoglucanase | 0.999Y | 664.29 | 12 | 621.5 | 13 | 588.23 | 9 |
| BC1G_01204.1 | Glyoxal oxidase | 0.983Y | 347.32 | 6 | 156.57 | 2 | 366.68 | 5 |
| BC1G_00455.1 | Mannosyl-oligosaccharide alpha-1,2-mannosidase precursor | 0.998Y | 1016.64 | 29 | 950.03 | 29 | 593.02 | 12 |
| BC1G_02314.1 | Extracellular phytase | 0.995Y | 460.62 | 12 | 230.08 | 4 | 427.18 | 14 |
| BC1G_10486.1 | Glutaminase GtaA | 0.992Y | 475.9 | 11 | 465.49 | 14 | 380.16 | 11 |
| BC1G_11266.1 | Laccase | 0.999Y | 550.72 | 17 | 418.69 | 14 | 565.69 | 16 |
| BC1G_10329.1 | Laccase | 0.989Y | 266.26 | 4 | 497.02 | 8 | 186.98 | 4 |
| BC1G_11835.1 | Phytase | 0.978Y | 84.38 | 1 | 61.01 | 3 | 191.12 | 4 |
| BC1G_02965.1 | Acid phosphatase/phosphoesterase | 0.996Y | 354.4 | 5 | 310.9 | 6 | 100.75 | 2 |
| BC1G_02163.1 | Ceratoplatanin | 0.996Y | 238.17 | 8 | 238.86 | 8 | 237.05 | 9 |
| BC1G_07521.1 | Aspartate protease | 0.999Y | 413.97 | 13 | 318.55 | 15 | 417.04 | 8 |
| BC1G_03710.1 | Carboxypeptidase S1 | 0.989Y | 52.47 | 1 | 58.78 | 1 | 79.96 | 1 |
| BC1G_01393.1 | Hypothetical protein | 1.000Y | 461.2 | 14 | 486.53 | 14 | 510.93 | 9 |
| BC1G_15201.1 | Hypothetical protein | 1.000Y | 240.06 | 10 | 211.51 | 14 | 329.78 | 13 |
| BC1G_12279.1 | Hypothetical protein | 1.000Y | 41.65 | 1 | 47.4 | 1 | 66.02 | 3 |
| BC1G_12374.1 | Hypothetical protein | 0.999Y | 207.74 | 5 | 189.88 | 4 | 358.51 | 8 |
| BC1G_08393.1 | Hypothetical protein | 0.999Y | 150.34 | 3 | 258.01 | 3 | 155.89 | 3 |
| BC1G_14136.1 | Hypothetical protein | 0.999Y | 36.38 | 1 | 128.38 | 2 | 60.58 | 3 |
| BC1G_11139.1 | Hypothetical protein | 0.999Y | 61.09 | 2 | 72.08 | 3 | 72.26 | 1 |
| BC1G_00198.1 | Hypothetical protein | 0.999Y | 1004.38 | 21 | 736.07 | 15 | 1005.62 | 29 |
| BC1G_08801.1 | Hypothetical protein | 0.982Y | 199.37 | 5 | 86.34 | 1 | 51.09 | 1 |
| BC1G_10095.1 | Hypothetical protein | 0.951Y | 156.41 | 2 | 48.69 | 1 | 76.79 | 1 |
| BC1G_13215.1 | Hypothetical protein | 0.820Y | 144.78 | 2 | 113.98 | 1 | 122.66 | 2 |
a
Protein identification number provided by the broad institute B. cinerea BO5.10 database
b
Name/function was assigned based on sequence similarity when blasted using NCBI non redundant database
c
Signal P prediction value using algorithm SignalP3.0 server (http://www.cbs.dtu.dk/services/SignalP/)
d
Protein score calculated by adding individual non redundant peptide Mascot score over a threshold score at 1% False Discovery Rate as calculated by ProValT
e
Total number of spectra matched to proteins in all replicates in a growth condition of B. cinerea