Table 2.
Results of the pectinases identified in the current study.
| Gene Ida | Putative Functionb | Signal Pc | PE pectin | PE pectin | HE pectin | HE pectin | sucrose | sucrose |
|---|---|---|---|---|---|---|---|---|
| Scored | Spectrae | Scored | Spectrae | Scored | Spectrae | |||
| BC1G_00617.1 | Pectin methylesterase | 1.000Y | 247.49 | 6 | 296.65 | 11 | 122.37 | 2 |
| BC1G_03464.1 | Rhamnogalacturonase | 1.000Y | 59.08 | 1 | 142.64 | 8 | - | - |
| BC1G_13367.1 | Endopolygalacturonase 2 | 1.000Y | 179.84 | 6 | 53.75 | 2 | - | - |
| BC1G_09000.1 | Pectate lyase | 1.000Y | 141.08 | 3 | 160.45 | 2 | - | - |
| BC1G_12517.1 | Pectate lyase, putative | 1.000Y | 44.42 | 1 | 55.61 | 1 | - | - |
| BC1G_01617.1 | Exo-polygalacturonase | 0.996Y | 1098.98 | 29 | 833.38 | 33 | 116.36 | 2 |
| BC1G_07052.1 | Pectate lyase | 0.997Y | - | - | 384.47 | 9 | - | - |
| BC1G_00240.1 | Similar to exopolygalacturonase | 0.990Y | 389.71 | 5 | 143.36 | 2 | - | - |
| BC1G_13137.1 | Polygalacturonase | 0.986Y | 492.13 | 12 | 791.28 | 16 | 73.04 | 1 |
| BC1G_06840.1 | Pectin methylesterase | 0.232N | 332.97 | 8 | 436.87 | 13 | 363.34 | 7 |
| BC1G_07496.1 | Polygalacturonase | 0.000N | 106.26 | 2 | 230.48 | 5 | - | - |
| BC1G_00799.1 | Pectin methylesterase | 0.002N | 204.22 | 6 | 225.48 | 5 | - | - |
| BC1G_08033.1 | Polygalacturonase 6 | 0.999Y | 712.92 | 23 | 564.83 | 20 | - | - |
a
Protein identification number provided by the broad institute B. cinerea BO5.10 database
b
Name/function was assigned based on sequence similarity when blasted using NCBI non redundant database
c
Signal P prediction value using algorithm SignalP3.0 server (http://www.cbs.dtu.dk/services/SignalP/)
d
Protein score calculated by adding individual non redundant peptide Mascot score over a threshold score at 1% False Discovery Rate as calculated by ProValT
e
Total number of spectra matched to proteins in all replicates in a growth condition of B. cinerea