Skip to main content
. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Mol Cell. 2009 Oct 9;36(1):61–74. doi: 10.1016/j.molcel.2009.08.008

Figure 3. Epigenetic Characterization of the miR199a/214 Locus in Myoblasts and Myotubes.

Figure 3

ChIP-chip analyses of binding of the PRC2 subunits Suz12 and Eed, the PRC1 subunit Bmi1, and the H3K27 trimethyl mark (A) and the hypophosphorylated form of RNAP2 and the H3K4 trimethyl marker of transcriptional initiation (B) at the miR199a-2/214 locus in proliferating myoblasts; and the same factors in differentiated myotubes (C) along with analyses of the myogenic factors MyoD and Myog (D). (E) Real-time PCR analysis of Suz12 and Ezh2 mRNA (left panel) and immunoblot of Suz12 and tubulin (right panel) in C2C12 myoblasts transfected with siRNA against Suz12 (si-Suz12). (F) miR-214 and myogenin expression by real-time PCR in C2C12 cells transfected with si-Suz12. (G) Real-time PCR analysis of Suz12 and Ezh2 mRNA (left panel) and immunoblot of Ezh2 and tubulin (right panel) in C2C12 myoblasts transfected with siRNA against Ezh2 (si-Ezh2). (H) miR-214 and myogenin expression by real-time PCR in C2C12 myoblasts transfected with si-Ezh2. Error bars represent the standard deviations for n = 3 in (E, F, G, H), * p<0.05; ** p<0.005.