Table 1.

Chromosomal targeting of BAC vector and BAC reporter constructs

Cell lines	GM639.3		GM639.6	167b3.1		GM847.7		166a11.1	Overall BAC
Input DNAs	pBACa SVpuroF	117B23 cFtRvSVP	117B23 cFtRvSVP	117B23 cFtRvSVP	183M22 cFhRvSVP	117B23 cFtRvSVP	183M22 cFtRvSVP	117B23 cFtRvSVP	targeting
Vector backbone	pBACe3.6	pBACe3.6	pBACe3.6	pBACe3.6	pTARBAC1	pBACe3.6	pTARBAC1	pBACe3.6
Total numbers of isolated clones	30	9	20	29	40	18	33	53	202
No. of clones with dual luciferases	∼	9	20	22	37	18	32	22	160
No. of clones examined by Southern analysisb	5	9	14	16	16	13	16	12	96
No. of clones with targeted integration	5	8	9	15	14	8	11	5	70
Targeting efficiencies among luciferase-positive clonesc	∼	89%	64%	94%	88%	62%	69%	42%	73%
Targeting efficiencies among all clonesd	100%	89%	64%	71%	81%	62%	67%	17%	58%

^aA plasmid of 13.4 kb.

^bOnly a subset of luciferase-expressing clones were examined by Southern analysis.

^cTargeting efficiencies = numbers of targeted integration/numbers of clones examined by Southern analysis.

^dTargeting efficiencies = efficiencies among luciferase-positive clones × number of clones with luciferases/total number of isolated clones.