Table 2.
Transcription from integrated and native hTERT promoters
| Acceptor lines (targeted clones) | Rluca | Fluca | Rluc/Fluc | Endogenous hTERT mRNAb |
|
|---|---|---|---|---|---|
| hTERT/18S | hTERT/CRR9 | ||||
| 166a11.1 (tel−)c | 13.2 ± 7.8 | 404 ± 137 | 0.04 ± 0.03 | 0.02 ± 0.01 | 0.07 ± 0.04 |
| #8 | 14.4 ± 0.4 | 187 ± 3 | 0.08 ± 0.00 | ||
| #15 | 9.5 ± 0.4 | 392 ± 1 | 0.02 ± 0.00 | ||
| #26 | 3.8 ± 0.7 | 401 ± 2 | 0.01 ± 0.00 | ||
| #27 | 13.1 ± 0.3 | 547 ± 10 | 0.02 ± 0.00 | ||
| #47 | 25.1 ± 0.4 | 492 ± 6 | 0.05 ± 0.00 | ||
| 167b3.1 (tel+)c | 944 ± 575 | 559 ± 112 | 1.63 ± 0.71 | 104 ± 10 | 720 ± 71 |
| #1 | 720 ± 47 | 520 ± 38 | 1.39 ± 0.09 | ||
| #2 | 1968 ± 40 | 687 ± 1 | 2.86 ± 0.06 | ||
| #3 | 589 ± 13 | 483 ± 13 | 1.22 ± 0.03 | ||
| #4 | 697 ± 39 | 439 ± 25 | 1.59 ± 0.09 | ||
| #10 | 746 ± 27 | 668 ± 33 | 1.12 ± 0.04 | ||
| GM847.1 (tel−)c | 5.9 ± 2.7 | 484 ± 129 | 0.01 ± 0.00 | 0.02 ± 0.01 | 0.03 ± 0.01 |
| #26 | 4.0 ± 0.8 | 392 ± 3 | 0.01 ± 0.00 | ||
| #27 | 7.8 ± 2.1 | 576 ± 15 | 0.01 ± 0.00 | ||
| GM847.7 (tel−)c | 19 ± 5 | 476 ± 107 | 0.04 ± 0.02 | ||
| #5 | 12 ± 1 | 628 ± 40 | 0.02 ± 0.00 | ||
| #6 | 21 ± 2 | 437 ± 13 | 0.05 ± 0.01 | ||
| #7 | 24 ± 2 | 338 ± 10 | 0.07 ± 0.01 | ||
| #18 | 16 ± 1 | 519 ± 28 | 0.03 ± 0.00 | ||
| #19 | 20 ± 4 | 458 ± 5 | 0.04 ± 0.01 | ||
| GM639.3 (tel+)c | 93 ± 35 | 279 ± 48 | 0.33 ± 0.10 | 62 ± 3 | 75 ± 4 |
| #1 | 67 ± 7 | 241 ± 22 | 0.28 ± 0.03 | ||
| #2 | 69 ± 6 | 221 ± 6 | 0.31 ± 0.03 | ||
| #4 | 119 ± 2 | 281 ± 13 | 0.42 ± 0.01 | ||
| #5 | 67 ± 12 | 331 ± 12 | 0.20 ± 0.04 | ||
| #8 | 142 ± 8 | 318 ± 17 | 0.45 ± 0.02 | ||
| GM639.6 (tel+)c | 233 ± 96 | 791 ± 55 | 0.29 ± 0.12 | ||
| #9 | 387 ± 17 | 834 ± 25 | 0.46 ± 0.02 | ||
| #10 | 194 ± 33 | 790 ± 126 | 0.25 ± 0.04 | ||
| #11 | 145 ± 17 | 857 ± 44 | 0.17 ± 0.02 | ||
| #25 | 176 ± 8 | 744 ± 24 | 0.24 ± 0.01 | ||
| #26 | 261 ± 10 | 730 ± 45 | 0.36 ± 0.01 | ||
The bold numbers in these rows are averages of the numbers below.
aRelative levels of luciferase activities were normalized to amounts of live cells as determined by MTT assays.
bRelative levels of endogenous hTERT mRNA in parental immortal lines were determined by reverse transcription and quantitative PCR, normalized to either 18S ribosomal RNA or CRR9 mRNA.
cAverage luciferase activities in targeted clones at the same acceptor line.