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. 2009 Jul 23;37(17):5602–5609. doi: 10.1093/nar/gkp611

Figure 3.

Figure 3.

Screening of DNA polymerases for single-nucleotide insertion. (A) The reaction for ImON:NaNO base pair. (B) The reaction for ImNO:NaON base pair. All reactions used the primer-template combination 5′-FITC-GTTCTGGATGGTCAGCGCAC-3′ (20mer) and 3′-CAAGACCTACCAGTCGCGTGXGAACGGGTG-5′ (30mer, X = ImON, NaNO, ImNO or NaON). Lanes 1 and 14 indicate the 20-mer primer; lanes 2, 8, 15 and 21 indicate the results of KF (exo−); lanes 3, 9 16, and 22 indicate those of Taq DNA polymerase; lanes 4, 10, 17 and 23 indicate those of Tth DNA polymerase; lanes 5, 11, 18 and 24 indicate those of Vent (exo−) DNA polymerase; lanes 6, 12, 19 and 25 indicate those of Deep Vent (exo−) DNA polymerase; and lanes 7, 13, 20 and 26 indicate those of KOD Dash DNA polymerase.