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. 2009 Jul 23;37(17):5602–5609. doi: 10.1093/nar/gkp611

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Primer extension reactions by family B DNA polymerases. (A) The reaction for ImO^N:NaN^O base pair. (B) The reaction for ImN^O:NaO^N base pair. All reactions used the primer-template combination 5′-FITC-GTTCTGGATGGTCAGCGCAC-3′ (20mer) and 3′-CAAGACCTACCAGTCGCGTGXGAACGGGTG-5′ (30mer, X = ImO^N, NaN^O, ImN^O or NaO^N). The reaction was carried out in the presence of dYTP (NaN^OTP, ImO^NTP, NaO^NTP or ImN^OTP) and natural dNTPs. Lane 1 indicates the 20-mer primer; lane 2 indicates the control 30mer prepared by primer extension reaction (X = T); lanes 3, 5, 7 and 9 indicate the results of Vent (exo⁻); and lanes 4, 6, 8 and 10 indicate those of Deep Vent (exo⁻) DNA polymerase.