Figure 4.

YY1 KD effects during early embryogenesis. Blastocysts were isolated through crossing the female F1 mice (pSico-YY1, Zp3-Cre) with littermate breeders. The isolated blastocysts were categorized based on their genotypes and genders. The DNA from each category of blastocysts was first treated with the bisulfite conversion, followed by the restriction digestion-based COBRA (A) and sequencing (B). The digested PCR products for H19, Nespas and Peg3 are derived from male blastocysts, while Xist is shown in four categories due to the fact that X chromosome number varies depending on gender. The PCR products were digested by the following restriction enzymes: H19 was digested by ClaI, while Nespas, Peg3 and Xist were digested by TaqI. The sequencing results for Xist are from female blastocysts (Con and KD).