Figure 5.

PTCD1 knockdown leads to an increase in mitochondrial and nuclear-encoded subunits of Complexes I and IV. Mitochondria were isolated from cells transfected with PTCD1 siRNA, NT siRNA, pPTCD1, pEYFP and from untransfected cells. Mitochondrial proteins were resolved by SDS–PAGE and specific proteins were detected by immunoblotting. (A) COXI, COXII, COXIV and porin. (B) ND1 and porin (C) NDUFA9 and porin. The data are typical of results repeated on four separate mitochondrial preparations (one of which is shown in the supplementary Figure 6).