Fig. 5.

Migration potential of T-ICs. (A) High expression of c-Met in GFP^high cells. Total RNA was purified from GFP^high and GFP^low cells isolated from three independent original tumors and c-Met mRNA levels were evaluated by RT-PCR. β-actin, control. (B) c-Met is phosphorylated in T-ICs. Serial sections of one of the single cell-derived tumors were subjected to H&E (i and ii); anti-GFP (iii and iv); and anti-phospho-c-Met (v and vi) staining. Magnified views of the areas indicated by the squares in i, iii and v are shown in ii, iv, and vi, respectively. (Scale bars: i, iii, v, 1 mm; ii, iv, vi, 200 μm.) (C) Migration of T-ICs in a collagen gel. Dissociated cells from a tumor were inoculated into either a plain collagen gel (control) or a collagen gel containing HGF (10 ng/mL) and incubated for 4 days. Representative GFP⁺ tumor cells with and without HGF in a collagen gel are shown. (Scale bars: 50 μm.)