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. 2009 Sep 28;106(40):17031–17036. doi: 10.1073/pnas.0905299106

Fig. 3.

Fig. 3.

Inhibition of IL-1α suppresses the IL-6/IL-8 cytokine response. (A) HCA2 cells were infected with shGFP- or shIL-1α-expressing lentiviruses, selected, and expanded. Total mRNA was collected and transcripts for IL-1α and GAPDH were quantified by RT-PCR. (B) Cells infected with shGFP or shIL-1α-expressing lentiviruses were either untreated or treated with bleomycin and allowed to recover for 6 d. CM were collected over the next 24 h and analyzed for IL-6 (black bars) or IL-8 (gray bars) by ELISA. Levels were normalized to those found in untreated shGFP control cells. (C) Nuclear extracts were prepared from presenescent (Pre) and bleomycin-induced senescent (Sen) cells expressing shGFP or shIL-1α and analyzed for NF-κB (p65) and C/EBPβ activities using TransAM DNA binding ELISAs.