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Recombinant IL-1α complements IL-1α deficient senescent cells. (A) HCA2 cells infected with shGFP or shIL-1α-expressing (#1 and #3) lentiviruses were treated with bleomycin and allowed to recover for 6 d. Recombinant IL-1α (10 ng/ml) was added over the next 24 h and CM were collected for IL-6 assessment by ELISA. (B) CM collected for 24 h from IL-1α- or IL-1β-expressing HCA2 cells were analyzed for IL-6 secretion by ELISA.
