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CM from IL-1α deficient cells fails to support cancer cell invasiveness. The Boyden chamber invasion assay was used to determine the effects of CM from senescent HCA2 cells infected with shGFP or shIL-1α-expressing (#1 and #3) lentiviruses on the invasiveness of MDA-MB-231 human breast cancer cells. Data are presented as relative invasiveness of the cells through the Matrigel, where the levels are normalized to invasiveness stimulated by CM from shGFP control cells.
