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. 2009 Oct 6;106(40):17229–17234. doi: 10.1073/pnas.0909229106

Fig. 2.

Fig. 2.

ISE1 encodes a conserved, plant-specific DEAD-box RNA helicase. (A) Gene structure of ISE1. ISE1 mRNA (white box) contains a single exon (black) with a 553-bp intron in the 5′ UTR. The relative positions of the ise1–1 single amino acid mutation and the T-DNA insertions in ise1–2 and ise1–3 are indicated. The ISE1 protein consists of a 441-aa DEAD-box RNA helicase domain and a unique 110-aa N-terminal domain. (B) ISE1 contains all of the domains conserved throughout the DExD family of RNA helicases. Asterisk indicates site of the ise1–1 mutation. (C) Homologs of ISE1 are found throughout the green plants and the unicellular algae Ostreococcus tauri. The most similar protein to ISE1 in yeast and animals is eukaryotic initiation factor 4a, although there is no shared homology outside the RNA helicase domains. There are two isoforms of ISE1 in Nicotiana benthamiana.