Fig. 2.

Immunofluorescence quantification of GCP. Horizontal axis, FI ratio (growth cone/distal axon), Longitudinal axis, area ratio (growth cone/distal axon). The horizontal axis shows the GC accumulation index (a ratio of 1.0; the vertical black dotted line indicates that a given protein is evenly distributed in the distal axon versus the growth cone). The FI ratio for GAP-43 (Gap43; 1.315) is shown as a blue dotted line. Proteins in the upper right region of the graph are more concentrated in the growth cone than in the axon. Many of these are actin-binding proteins and proteins involved in vesicular trafficking. The FI ratio of each protein was tested by using the Kruskal-Willis statistical test (46) with GAP-43 as the comparison point. The proteins were grouped based on the two-sided 95% confidence interval, i.e., was it higher than, similar to, or less than that of GAP-43 (shown in red, blue, and black, respectively). Note that as expected, F-actin (as detected with rhodamine phalloidin; in red), which is concentrated in the growth cone, and β-tubulin (green), which is at higher levels in the axon than in the growth cone, are distant from each other on the graph. See Table S4 for detailed information.