Fig. 5.

Thiol reductase activity of pERp1 in vitro. Activity was assayed by using 140 mM insulin as substrate and 1 μM (Left) or 10 μM (Right) pERp1 and, for reference, E. coli DsbA and DsbC as catalysts. The onset of aggregation (increased optical density at 650 nm) is a measure for reductase efficiency.