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Intersubunit isopeptide bonds in SpaA. Fragmentation spectra of the parent ion at m/z 585.73+ containing the intersubunit bond between SpaA Lys-190 and Thr-494 are shown. Ion types are indicated, and internal ions are shown in italics. The structure of crosslinked peptide is shown above the spectra. Daughter ions produced during MS/MS of these peptides are summarized in Table S4.
