Fig. 1.

Characterization of pERp1. (A) Amino acid sequence of mouse pERp1. The peptide sequences obtained from mass spec analysis are underlined. The 6 conserved cysteine residues are marked with asterisks, and the CXXC motif is in bold. The signal sequence is italicized, and the ER retention signal is double underlined. (B) Tissue distribution of pERp1 mRNA. A human multitissue Northern blot was probed with pERp1 or β-actin cDNA. The transcripts were detected by autoradiography. β-actin was used as a loading control. 1, peripheral blood leukocyte; 2, lung; 3, placenta; 4, small intestine; 5, liver; 6, kidney; 7, spleen; 8, thymus; 9, colon (no mucosa); 10, skeletal muscle; 11, heart; 12, brain.