Fig. 1.

Spleen and lymph node weights in LMP1⁺TRAF5^+/+ and LMP1⁺TRAF5^−/− mice. Spleens and lymph nodes were harvested from 3- to 4-month-old LMP1⁻TRAF5^+/+ (open squares), LMP1⁺TRAF5^+/+ (closed triangles), and LMP1⁺TRAF5^−/− (open triangles) mice and weighed. To account for variability in mouse size, spleen weights are represented as a percentage of overall body weight (A). Cervical, axillary, and brachial lymph nodes were harvested and weighed, and are represented as average lymph node weight (B). Purified splenic B cells from LMP1⁺TRAF5^−/− mice and littermate controls were lysed in 2× SDS lysis buffer and separated by SDS/PAGE. TRAFs, mCD40-LMP1, and actin were detected by Western blot (C). Surface expression of mCD40-LMP1 on purified splenic B cells was detected by using an anti-mCD40-FITC mAb or isotype control mAb for staining by immunofluorescence flow cytometry (D).