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TAK1 and Akt activation in B lymphocytes from LMP1+TRAF5+/+ and LMP1+TRAF5−/− mice. Purified B cells were stimulated in vitro with 10 μg/mL anti-mCD40 (HM40.3) or 10 μg/mL isotype control for 10–90 min and used in a phospho-TAK1 Western blot (Upper), or stimulated in vitro with 10 μg/mL anti-mCD40 (HM40.3), 10 μg/mL isotype control, or 100 ng/mL CpG-B (2084) for 10–90 min and used in a phospho-Akt Western blot (Lower).
