Figure 4.

(A) Resveratrol, apocynin and genetic deletion of TNFα significantly reduced aortic O₂^.− production in Lepr^db. Incubating aortas of m Lepr^db with TNFα increased O₂^.− production. White arrow indicates the lumen of aorta. (B) and (C), Resveratrol significantly decreased NAD(P)H oxidase activity and gp91^phox protein expression in Lepr^db. NAD(P)H oxidase activity was lower in db^TNF−/db^TNF− than in Lepr^db (B). n=4 separate experiments. *P < 0.05 vs. m Lepr^db; #P<0.05 vs. Lepr^db.