Skip to main content
. Author manuscript; available in PMC: 2009 Oct 13.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Dec 3;366(1):104–109. doi: 10.1016/j.bbrc.2007.11.091

Figure 3. Selective activation of PKA Type I inhibits TNF-α mRNA levels in both J774 and Raw 264.7 macrophages.

Figure 3

A, C) Synergistic combinations of cAMP analogs (250 μM) were used to activate PKA type I or PKA type II in Raw (A) and J774 (C) macrophages. B, D) The addition of the Epac activator 8-CPT-2-OME did not alter inhibition of TNF-α mRNA levels by PKA type I or PKA type II activator combinations in Raw (B) or J774 (D) cells. E) J774 macrophages were induced with LPS (1 μg/mL) for 1 h with increasing concentrations of Sp-8-Br-cAMPS and/or Rp-8-Br-cAMPS. Macrophages were pretreated for 30 min with cAMP analogs prior to the addition of LPS (1 μg/mL).