. Author manuscript; available in PMC: 2010 Sep 1.

Published in final edited form as: Biochemistry. 2009 Sep 1;48(34):8271–8278. doi: 10.1021/bi900777s

Table 6.

Incorporation of natural nucleotides and analogues by BF across from both natural and analogue template nucleosides.

dNTP	Template	V_max (%/min⁻¹)	K_M (µM)	k_cat/K_M (µM⁻¹ min⁻¹)
dCTP	G	19 ± 6.0	1.8 ± 0.5	10.4

	A	21 ± 2	8.2 ± 6	2.6
dTTP	P	62 ± 8	330 ± 90	0.19
	7DA	38 ± 8	38 ± 15	1.0
	7DP	8.4 ± 0.1	6.4 ± 0.4	1.3

	A	1.1 ± 0.4	390 ± 300	2.9 × 10⁻³
dGTP	P			N/A ^a
	7DA			N/A
	7DP			N/A
	C	24 ± 2	19 ± 6	1.3

dATP	T	12.1 ± 0.6	2.2 ± 0.5	5.5

dPTP	T	4.4 ± 0.2	6.7 ± 1	0.66

7DdATP	T	21 ± 1	56 ± 10	0.37
	G			N/A

7DdPTP	T	36 ± 4	160 ± 40	0.23
	G			N/A

1DdATP	G	1.7 ± 0.2	340 ± 70	5.0 × 10⁻³

N/A Incorporation was too low to accurately measure.