Table 7.
dNTP | Template | Vmax (%/min−1) |
KM (µM) |
kcat/KM (µM−1 min−1) |
---|---|---|---|---|
A | 3.1 ± 0.2 | 19 ± 4.4 | 0.16 | |
P | N/A a | |||
dITP | 7DA | 2.3 ± 0.2 | 260 ± 80 | 8.8 ×10−3 |
7DP | N/A | |||
C | 55 ± 4.0 | 79 ± 24 | 0.69 | |
dATP | I | 5.3 ± 0.4 | 130 ± 40 | 4.0 × 10−2 |
dPTP | I | N/A | ||
7DdATP | I | 4.8 ± 1 | 390 ± 170 | 1.2 × 10−2 |
7DdPTP | I | N/A | ||
1DdATP | I | 25 ± 1 | 480 ± 50 | 5.3 × 10−2 |
dCTP | I | 16 ± 0.4 | 3.5 ± 0.5 | 4.7 |
N/A Incorporation was too low to accurately measure.