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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biochemistry. 2009 Sep 1;48(34):8271–8278. doi: 10.1021/bi900777s

Table 7.

Incorporation of dNTPs opposite a templating hypoxanthine and dITP opposite various templating nucleosides by BF.

dNTP Template Vmax
(%/min−1)
KM
(µM)
kcat/KM
(µM−1 min−1)
A 3.1 ± 0.2 19 ± 4.4 0.16
P N/A a
dITP 7DA 2.3 ± 0.2 260 ± 80 8.8 ×10−3
7DP N/A
C 55 ± 4.0 79 ± 24 0.69

dATP I 5.3 ± 0.4 130 ± 40 4.0 × 10−2
dPTP I N/A
7DdATP I 4.8 ± 1 390 ± 170 1.2 × 10−2
7DdPTP I N/A
1DdATP I 25 ± 1 480 ± 50 5.3 × 10−2
dCTP I 16 ± 0.4 3.5 ± 0.5 4.7
a

N/A Incorporation was too low to accurately measure.