Figure 3.

Analysis of PARP–RNA complexes by gel retardation. ³²P-labeled RNA stem loop (20 fmol) synthesized from CFTR cDNA was incubated with various amounts of PARP for the indicated times at 30°C in 10 μl of reaction mixture and was analyzed by 6% acrylamide gel electrophoresis. For analysis in the presence of DNA breaks (DB) (λ-DNA–BstEII digest), PARP–RNA complexes were preformed (10-min incubation) and incubated for a further 10 min after the addition of DNA breaks.