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. Author manuscript; available in PMC: 2010 Nov 27.
Published in final edited form as: Regul Pept. 2009 Jul 17;158(1-3):149–155. doi: 10.1016/j.regpep.2009.07.008

Figure 3. Effect of suppressing PTH1R expression or neutralizing secreted PTHrP on LoVo cell apoptosis.

Figure 3

(A) PTH1R expression was suppressed by transfection with an siRNA targeting PTH1R. Two independent siRNAs (1 and 2) were used. As control, cells were transfected with a non-targeting control (NTC) siRNA. mRNA levels were measured 48 h after transfection. Values are expressed relative to the NTC value, set arbitrarily at 1.0. Each bar is the mean ± S.E.M. for three independent experiments. * = Significantly different from the NTC value (P < 0.001). (B) Apoptosis after transfection with siRNAs targeting PTH1R. Apoptosis was measured 48 h after transfection. Each bar is the mean ± SEM of three independent experiments for each of two independent siRNAs. * = Significantly different from the respective NTC control value (P < 0.001). (C) Apoptosis after neutralizing secreted PTHrP with an anti-PTHrP antibody. Cells were treated with an anti-PTHrP antibody (final concentration 2 µg/ml). Apoptosis was measured after 48 h. − = no treatment; IgG = IgG control. Each bar is the mean ± SEM of three independent experiments. * = Significantly different from the respective IgG control value (P < 0.001).