Figure 5. Effects of wild-type PTHrP and PTHrP deleted over the nuclear localization signal or signal peptide on anchorage-independent cell growth.
(A) Representative colonies after 2 weeks in culture. Magnification, ×10. (B) Each bar is the mean ± SEM of 20 colonies for each of three independent clones overexpressing WT PTHrP (WT), NLS-deleted PTHrP (ΔNLS), or SP-deleted PTHrP (ΔSP), or transfected with empty vector (V). * = Significantly different from the control (V) value (P < 0.001).