Table 1. Within-run precision studies using patient samples from East Africa, Abbott m2000rt RealTime™ HIV-1 assay with manual sample preparation compared with the ROCHE COBAS® AmpliPrep™/AMPLICOR™ HIV-1 MONITOR® v1.5a.
| Sample run number | ROCHE COBAS® AMPLICOR™ HIV-1 MONITOR® v1.5 | Abbott m2000rt RealTime™ HIV-1 |
|---|---|---|
| 1 (n=10) | ||
| Mean | 5.51 | 5.07 |
| Range (low, high) | (5.43, 5.55) | (4.84, 5.22) |
| SD (manufacturer's claim)b | 0.04 (0.1) | 0.12 (0.04) |
| CV | 0.71% | 2.27% |
| 2 (n=10) | ||
| Mean | 4.32 | 4.20 |
| Range | (4.19, 4.38) | (4.16, 4.24) |
| SD (manufacturer's claim)b | 0.08 (0.14) | 0.03 (0.02) |
| CV | 1.76% | 0.66% |
| 3 (n=10) | ||
| Mean | 3.07 | 3.29 |
| Range | (2.92, 3.31) | (3.15, 3.36) |
| SD (manufacturer's claim)b | 0.13 (0.17) | 0.06 (0.05) |
| CV | 4.31% | 1.85% |
a
Log transformed HIV-1 RNA values are reported
b
Standard deviation (manufacturer's claims for total assay variability)