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. Author manuscript; available in PMC: 2009 Oct 14.
Published in final edited form as: Cancer Biol Ther. 2009 Jul 30;8(14):1389–1401. doi: 10.4161/cbt.8.14.8880

Figure 5. PTEN negatively regulates the YY1 cofactor.

Figure 5

A. Whole cell lysates of 786-0 and A498 cells were immunoblotted for YY1 expression. Total protein was quantitated by Bradford Assay and equalized before loading. Actin was used as a loading control. Protein expression analysis was repeated independently three times. B. The 786-0 line was transiently transfected with either the empty vector, pcDNA3.1, or the pc/PTEN expression construct. Whole cell lysates were purified and immunoblotted for PTEN and YY1 protein expression. C. The A498 line was transfected with either control or PTEN-targeted SMARTpool® siRNAs [25nM] for 72hrs. Whole cell lysates were purified and analyzed for PTEN and YY1 protein levels by western blotting. A non-specific (NS) band was used as a loading control in B and C. Transfections were repeated three times in triplicate.