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. 1998 May 26;95(11):6175–6180. doi: 10.1073/pnas.95.11.6175

Figure 1.

Figure 1

Characterization of the tracers. (A) The derivatized Om as well as the native molecule have the same migration behavior in SDS/PAGE. Om-DNP (5 μg) in lane 1 and native Om (5 μg) in lane 2 were run at 150 V for 90 min. The minigels were stained with Coomassie blue R-250. (B) Immunoelectrophoresis on a 1% agarose gel shows that the net electric charge of Om-DNP (lane 1) is not significantly different from that of native Om (lane 2). The ampholytes used were pH 3–9, and the direction of pH gradient is indicated by the + and − signs. Every lane was loaded with 7 μg of protein. (C) SDS/PAGE of Om-DNP transferred to nitrocellulose paper (1 h at 700 mA) and blotted with anti-DNP antibody (diluted 1:2000). Only Om-DNP is recognized by the antibody (lane 1); the native Om in lane 2 does not give a signal. Each lane was loaded with 5 μg of protein.