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. Author manuscript; available in PMC: 2009 Oct 14.
Published in final edited form as: Oncogene. 2001 Aug 2;20(34):4629–4639. doi: 10.1038/sj.onc.1204680

Figure 3.

Figure 3

Coimmunoprecipitation of Myc-ER™ and endogenous c-Myc with anti-Max antibody. Whole cell extracts of CEM-C7-14 or C7-MycER™ cells treated with ethanol (C), 1 μM Dex (D), 250 nM 4HT (T) or Dex plus 4HT (DT) for 20 h were inmunoprecipitated with the polyclonal anti-Max antibody C-124. or non-specific antibody (P). Antibody bound proteins were purified on Protein A-Agarose beads and resolved by 10% SDS–PAGE and electroblotting. Whole cell extract (E) from C7-MycER™ cells was run in one lane as a control. The membrane was probed with the c-Myc monoclonal antibody Mycl-9E10.2, and developed by ECL reaction