Figure 4.
Survival likelihoods of neonatal CD-1 mice following oral challenge with wild-type O1 Ogawa V. cholerae O395. Three- to five-day-old pups (cohort size 15) were gavaged with 50 μl of a preparation containing 106 or 107 CFU of V. cholerae O395 mixed 1:1 with pooled day 56 serum from mice transcutaneously immunized with neoglycoconjugate (CHO-BSA) and immunoadjuvantative cholera toxin (CT) or CT alone. Challenged mice were kept at 30°C and monitored for death every 3 to 6 h starting 18 h after oral challenge till 48 h after oral challenge. Survival curves were compared by log rank testing.