Table 1. Summary of nuclear proteins identified from liver and heart tissue nuclear isolations by 1DE/MS/MS analysis.
| Nuclear Protein | Nuclear compartment | Liver | Heart | |
|---|---|---|---|---|
| Density | PE.a | PE. | ||
| Histone H1 | DNA binding | ✓b | ✓ | ✓ |
| Histone H2B | DNA binding | ✓ | ✓ | ✓ |
| Histone H2A | DNA binding | ✓ | -c | ✓ |
| Histone H4 | DNA binding | ✓ | ✓ | ✓ |
| Histone H3 | DNA binding | ✓ | - | - |
| DNA-directed RNA polymerases III | DNA binding | ✓ | - | - |
| DNA helicase II | DNA binding | ✓ | - | - |
| Lamin A | nuclear matrix | ✓ | - | - |
| Lamin B1 | nuclear matrix | ✓ | - | - |
| Cytokeratin 8 | nuclear matrix | ✓ | - | - |
| Cytokeratin 18 | nuclear matrix | ✓ | - | - |
| Brix domain containing 1 | nucleolus | ✓ | - | - |
| Fibrillarin | nucleolus | ✓ | - | - |
| hnRNP A2/B1 | hnRNPs/splicing factor | - | ✓ | - |
| hnRNP C | hnRNPs/splicing factor | ✓ | - | - |
| hnRNP M | hnRNPs/splicing factor | ✓ | - | - |
| Small nuclear ribonucleoprotein B | hnRNPs/splicing factor | ✓ | - | - |
| Splicing factor 3b, subunit 1 | hnRNPs/splicing factor | ✓ | - | - |
a)
PE. - ProteoExtract kit.
b)
‘✓’indicates that the protein is present in fraction.
c)
‘-’ indicates that protein was not detected in fraction