Table I.

RT-QPCR primers. Primer sets with oligo names and sequences used for the analysis of various transcripts (aldolase C, c-myc, CYP1A1, GREB1, LDH-A, pS2, VEGF, and the standard 18S) are given with forward (F) and reverse (R) orientations noted. Primer melting temperatures used during PCR analysis are shown along with the fragment sizes generated.

Aldolase C			OL88: 5′-ATAATGGTGTTCCCTTCGTCCGA-3′ (F)
(218 bp)			OL89: 5′-TGCAGAGGGTGTACGCTCACTG-3′ (R)
c-myc			OL230: 5′-GCCCCTCAACGTTAGCTTCA-3′ (F)
(150 bp)			OL231: 5′-TTCCAGATATCCTCGCTGGG-3′ (R)
CYP1A1			OL90: 5′-GGCCACATCCGGGACATCACAGA-3′ (F)
(336 bp)			OL91: 5′-TGGGGATGGTGAAGGGGACGAA-3′ (R)
GREB-1			OL213: 5′-CAAAGAATAACCTGTTGGCCCTGC -3′ (F)
(172 bp)			OL214: 5′-GACATGCCTGCGCTCTCATACTTA-3′ (R)
LDH-A			OL86: 5′-GCCCGACGTGCATTCCCGATTCCTT-3′ (F)
(361 bp)			OL87: 5′-GACGGCTTTCTCCCTCTTGCTGACG-3′ (R)
pS2			OL196: 5′-GCCCAGACAGAGACGTGTACA-3′ (F)
(172 bp)			OL197: 5′-TCACACTCCTCTTCTGGAGGG-3′ (R)
VEGF			OL118: 5′-GGGGGCTGCTGCAATGACG-3′ (F)
(441 bp)			OL119: 5′-CGCCTCGGCTTGTCACATCTG-3′ (R)
18S			OL96: 5′-CGCCCCCTCGATGCTCTTAG-3′ (F)
(377 bp)			OL97: 5′-CGGCGGGTCATGGGAATAAC-3′ (R)