Table (III).

Free energy values of the UHS and MHS in kcal/mol

		UHS						MHS
		TM ± SD		TR ± SD		SOL ± SD		TM ± SD		TR ± SD		SOL ± SD
polar	C	-0.07	0.09	0.56	0.03	-0.22	0.06	-0.01	0.01	0.14	0.14	-0.09	0.10
	N	0.37	0.03	-0.14	0.01	-0.10	0.01	0.41	0.04	-0.15	0.03	-0.12	0.04
	Q	0.31	0.05	-0.01	0.03	-0.19	0.03	0.60	0.09	-0.13	0.07	-0.19	0.04
	S	0.02	0.03	0.02	0.03	-0.04	0.02	0.13	0.28	-0.01	0.07	-0.06	0.14
	T	-0.01	0.01	0.02	0.01	0.00	0.01	-0.02	0.01	-0.04	0.04	0.06	0.04
charged	D	0.52	0.04	-0.08	0.03	-0.21	0.02	0.82	0.28	0.05	0.18	-0.34	0.05
	E	0.47	0.02	0.10	0.02	-0.31	0.01	0.84	0.05	0.06	0.10	-0.36	0.04
	K	0.69	0.03	-0.13	0.03	-0.22	0.03	0.99	0.15	-0.08	0.03	-0.30	0.04
	R	0.40	0.04	-0.11	0.02	-0.15	0.01	1.06	0.13	-0.22	0.08	-0.18	0.10
apolar	A	-0.10	0.02	0.07	0.02	0.05	0.01	-0.15	0.01	0.12	0.02	0.07	0.01
	G	-0.09	0.02	-0.06	0.01	0.19	0.02	-0.07	0.03	0.05	0.03	0.03	0.06
	I	-0.22	0.02	0.12	0.02	0.18	0.02	-0.24	0.01	0.13	0.08	0.23	0.07
	L	-0.16	0.01	0.06	0.02	0.14	0.02	-0.26	0.03	0.15	0.07	0.24	0.00
	M	-0.12	0.03	0.01	0.05	0.13	0.04	-0.07	0.04	-0.06	0.02	0.16	0.02
	P	0.34	0.04	-0.08	0.02	-0.15	0.02	0.36	0.07	-0.09	0.07	-0.14	0.09
	V	-0.16	0.01	0.15	0.02	0.06	0.01	-0.22	0.03	0.22	0.05	0.09	0.01
aromatic	F	-0.19	0.02	-0.02	0.02	0.34	0.01	-0.22	0.05	0.00	0.01	0.35	0.11
	H	0.29	0.03	-0.14	0.03	-0.07	0.04	0.55	0.41	-0.28	0.03	0.03	0.12
	W	0.08	0.02	-0.19	0.02	0.19	0.03	0.07	0.11	-0.24	0.15	0.36	0.24
	Y	-0.07	0.02	0.04	0.01	0.03	0.02	0.20	0.09	-0.13	0.04	-0.02	0.12

The table shows the knowledge-based values for the free energies in kcal/mol and their corresponding standard deviations (SD) for the 20 amino acids in the three regions of the membrane bilayer (TM), the transition region (TR) and the soluble region (SOL) for both scales, the UHS and the MHS. The shaded cells indicate the preference of the amino acid for that region. Note that Serine and Threonine in the UHS show almost no preference for any of the three regions.