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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Microbiol. 2009 Jul 9;73(3):434–445. doi: 10.1111/j.1365-2958.2009.06782.x

Figure 1. Effect of mutations in the RetS/GacS/A/RsmY/Z/A pathway on expression of a T3S gene exoS and on development of biofilm.

Figure 1

A. Miller assays were used to measure expression of the exoS-lacZ fusion in wild type and mutant strains of P. aeruginosa. As induction of the T3S genes occurs under conditions of low calcium, cultures were grown in Ca2+-depleted (grey bars) and Ca2+-replete (white bars) LB media, at 37°C for 4 hours.

B. Biofilm phenotypes were measured in wild type and mutant strains of P. aeruginosa as the attachment of bacteria to tubes and formation of biofilm rings and pellicles after 14 and 20 h static incubation at 30°C.